克雷伯氏肺炎菌莢膜型K47菌株之噬菌體分離 及其莢膜多醣分解酶表現 Investigating the Feasibility of Creating a Sustainable Microbial Fuel Cell Using Symbiotic Fungi and Algae from Lichens
Klebsiella pneumoniae (KP) has become increasingly prevalent in Asia recently, posing not only a threat to public health but also a significant burden on healthcare economics. Due to the widespread use of antibiotics in the early treatment of this infection, KP has gradually developed antibiotic resistance, rendering many antibiotic therapies less effective. As a result, finding alternative therapies has become a key focus in recent years. Among these, bacteriophages and their derivative proteins are considered promising candidates for the development of antibiotic alternatives.
In this study, a series of experiments were conducted on several candidate bacteriophages potentially carrying capsular polysaccharide depolymerases. These bacteriophages were tested for their bactericidal effects on the capsular K47 strain. After whole-genome sequencing of the bacteriophages was performed, and experiments will be made to express the potential genes with capsular polysaccharide depolymerase.
In the future, if we successfully express and purify sufficient amounts of the target protein, we will test its activity against the K47 KP capsule. Through this work, we aim to contribute to the fight against Klebsiella pneumoniae.