sRNA plays a crucial role in sperm function across various species. In Caenorhabditis elegans, the absence of the sperm-related sRNA , ALG-3/4 26G sRNA, results in infertility at 25°C. Additionally, IFE-1, a homologue of the human eukaryotic translation initiation factor EIF4E, is primarily expressed in the male germline. Previous studies show that ‘defects in IFE-1’ or ‘the lack of sperm-related sRNA’ both lead to defective spermatids. Given the similarity in their effects, we hypothesize that IFE-1 is involved in the pathway of 26G sRNA generation. Specifically, we propose that IFE-1 assists the enzyme NYN-3 in recognizing the msd-1 template and cleaving its mRNA to promote 26G sRNA production. Our research explores the relationship between IFE-1 and MSD-1::GFP, revealing that under normal IFE-1 conditions, high temperatures do not affect MSD-1::GFP expression. Furthermore, since GFP is only expressed in male sperm, we infer that msd-1:gfp functions only in male sperm. The potentially positive regulation of MSD-1::GFP expression by 26G sRNA offers a new therapeutic approach distinct from traditional sRNA-based genetic treatment.