Dengue fever is transmitted by the Aedes aegypti mosquito. In recent years, the distribution of A. aegypti has been expanding, leading to the deaths of millions. Vector control is crucial for preventing disease transmission. However, there are currently no safe and effective vaccines or treatments. Overreliance on insecticides has led to resistance in the vector mosquitoes and environmental concerns, making the development of alternative strategies a priority. This study has developed a novel insecticidal approach using RNA interference (RNAi) targeting the ADAM12d gene in the mosquito. The impact on the ADAM12d expression was assessed using quantitative reverse transcription PCR (qRT-PCR). Following gene cloning, large quantities of double-stranded RNA (dsRNA) were successfully replicated using bacterial vectors. Introducing this dsRNA into the habitats of A. aegypti larvae led to the suppression of ADAM12d gene expression. Results indicated that this RNAi insecticide caused molting abnormalities and slowed growth in the larvae, increasing their mortality rate, thereby reducing the proliferation and breeding of the vector mosquitoes. Practical application studies have also commenced. The findings of this study will contribute to understanding the biological function of ADAM12d and highlight its potential as a new biological control method. This RNAi approach is specific, not affecting other mosquito species, and directly targets the disease-transmitting mosquitoes. Therefore, it aligns well with ecological considerations and current needs, showing promise as an effective novel control agent.