In recent clinical observations, we found that there are many coexisting microorganisms in the tumor microenvironment. This study found through bioinformatics that the co-metabolite 2'O-methyluridine between microorganisms and the human body seems to have the effect of promoting the growth of breast cancer cell lines. Experiments were designed to further analyze the actual mechanism of action between microorganisms and breast cancer cell lines.
This study explores the mechanism of action of 2'O-Methyluridine on two common breast cancer cell lines, MCF-7 and T47-D, by using real-time cell analysis, cell colony formation assays, real-time polymerase chain reaction and Western blotting. We revealed that 2'O Methyluridine affected the growth of T47-D differently under different circumstances, while the growth of MCF-7 was inhibited and the expression of the gene encoding the mitochondrial ATP synthase subunit in breast cancer cells decreased.
The research results of this experiment can provide a new direction for breast cancer treatment. If microbial metabolites have different responses to different types of cancer cell lines, we can analyze the patient's tumor cells in advance and treat the microorganisms and cancer cells at the same time. It may be possible to achieve better therapeutic effects and reduce the side effects of chemotherapy.