Using fiber threads made from the common Taiwanese plant "Ramie" for microfluidic applications, a low-cost, user-friendly, and highly accurate module has been developed to assess factory pollution concentrations. Concentration measurements of Lead ions, Copper ions, Thiocyanate, Phosphate, Methylene blue, and Congo red have been conducted using the line-based microfluidic system. Through line-based microfluidic, these pollutants commonly generated in industrial processes or the textile industry have been detected, with concentrations ranging from a maximum of 2.0 g/L to a minimum of 0.4 g/L. The coefficient of determination (R²) exceeds 0.98, providing a complete calibration curve. This module enables highly accurate concentration detection of pollution. Furthermore, our reserach explores various numerical regressions exhibited by the Ramie line-based microfluidics with different analytes. In addition to linear regressions, logarithmic regressions have also been observed. Through viscosity experiments, the relationship between microfluidic concentration and chromatographic length has been investigated. This method allows for the rapid establishment of calibration curves to determine the presence of analytes.